?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.relation=http%3A%2F%2Fmiis.maths.ox.ac.uk%2Fmiis%2F198%2F&rft.title=Measuring+Plant+Genetic+Diversity+Using+Inter-Simple+Sequence+Repeats+(ISSRS)&rft.creator=Iannetta%2C+Pietro&rft.subject=None%2FOther&rft.description=Simple+sequence+repeats+(SSRs)+have+great+utility+as+they+are+conserved+and+present+in+all+eukaryotic+genomes.+Here+we+report+the+use+of+a+simple+PCR+with+fluorescently-labelled+primers+to+amplify+inter-SSR+markers+(ISSRs)+for+diversity+assessments.+The+use+of+ISSR+markers+does+not+rely+upon+specific+genetic+sequence+information%2C+or+prolonged+method+development+and+may+be+measured+rapidly+using+the+automated+equipment.+The+major+restriction+of+the+ISSR+method+is+at+the+analysis+stage%2C+as+the+markers+are+dominant+it+is+not+possible+to+distinguish+heterozygotes+as+loci.+We+obtained+ISSR+data+from+ca.+60+phenotypically+characterised+Capsella+bursa+pastoris+L.+Medic+(shepherds+purse)accessions+that+had+been+isolated+from+a+diverse+mix+of+arable+field+sites+throughout+the+UK.+We+developed+mathematical+scripts+for+use+with+the+free+statistical+software+tool+R+(http%3A%2F%2Fwww.rproject.org%2F)%2C+that+processed+the+molecular+data+in+a+binary+format+to+estimate+genetic+diversity+(using+the+Jaccard+co-efficient)%2C+and+that+related+genotype+to+the+plant+phenotypic+and+environmental+(site+specific)+traits.+The+methodology+established+has+the+power+to+predict+the+relationship+between+environmental+and+plant+morphological+characteristics.&rft.date=2007-12&rft.type=Study+Group+Report&rft.type=NonPeerReviewed&rft.format=application%2Fpdf&rft.language=en&rft.identifier=http%3A%2F%2Fmiis.maths.ox.ac.uk%2Fmiis%2F198%2F1%2Freport2.pdf&rft.identifier=++Iannetta%2C+Pietro++(2007)+Measuring+Plant+Genetic+Diversity+Using+Inter-Simple+Sequence+Repeats+(ISSRS).++%5BStudy+Group+Report%5D+++++